Our Patented dual-piston Differential Displacement Pipettors are a new core liquid-handling invention that can accurately pipette samples down to nanoliters and deliver even the tiniest samples contact-free. Pipetting, the commonest lab test in the world, with millions of manual and automated robotic diagnostics daily, has unstable aspiration and hanging drop delivery for under 10 µL. Solved with new disruptive technology, small barrier to constructive entry.

Two robust pistons of slightly different diameters, bottom a tube and top that may be tapered, line up facing in the same chamber. Moving together, sealing tightly gives fine resolution from the cross-section difference to aspirate nanoliters accurately. Hand unit typical diameter difference is 0.005 inches, top 0.156″ and bottom 0.161″. Moving together gives resolution as fine as 50 µL syringe, or even 10 µL if top is 0.160″ or tapered, handling range 0.1 µL to 20 µL with under 0.5 inch vertical movement. 1 piston alone gives high flow like a 1 mL syringe, easily delivering over 1.5 meters/second TEVIA (Tip Escape Velocity in Articulatum) to blow a small sample with height in tip at least 4X tip ID completely off to the destination without touching.

Covered by US patents 11491477, 11813606 and 11951470. One unit giving both fine differential resolution and high flow power! 1 µL CV is 2% and accuracy 3-4%, with standard deviation of 0.02 µL across the whole range.

96-channel Differential Pipetting system setup for 0.25 µL to 10 µL pipetting. Piston diameters 0.184″ and 0.187″ – fine Differential Aspiration like a 34 µL syringe with full 2 mm stroke per µL, and single piston up to 40 µL with flow like a 1.1 mL syringe to blow off cleanly. A 30-fold resolution multiplier. With 50 µL Labcon 8010 tips. Base assembly with downward reaching steel mandrels grips way down inside each tip so that only about 10 µL of air remains.

Each such setup calibrated by runs showing true regression of theoretical vs expected volume, here a slope of 1.045 and Y intercept of – 429 nanoliters, or algorithm Observed volume = 1.045 (theoretical) – 429 nanoliters to call out exact excursion needed. Our program NB250-2000GRAD-11D-0 aspirates 96 samples and delivers them to 384 plate in 4 quadrants, a favorite being 0.5 µL, 1µL, 1.5 µL and 2 µL that show a perfect visual size pattern of all 384.

Covers 10 µL to 0.25 µL with SD 0.02 µL. Differential aspiration solid for several nanoliters but future work will show if finer probes permit clean blow off or if touchoff delivery needed.