Differential Pipettor Dispenser Diluter Manual

1. Setup & Connections
  • Left (blue) connector: Connect tubing to the reservoir
  • Right (clear/green) connector: Connect tubing to the probe
  • Connect the probe electrically and power on the instrument (rear panel)

The pump will initialize and display “Ready”

2. Priming the System
  1. Press the Prime Pump button
  2. The system will prime automatically
3. Selecting a Program
  1. Press Select
    • Display shows: first step of Program #1 (e.g., Disp 100.0 µL)
  2. Press Select again to scroll through programs
    • OR enter the program number directly using the keypad
4. Running a Program
  1. Attach a new disposable tip to the hand probe
  2. Press and release the probe switch to run each step
Indicators:
  • Green LED OFF: Step in progress
  • Green LED ON: Ready for next step
  • Beep sound:
    • Signals step completion
    • Indicates when probe can be moved
5. After Each Step
  • The display shows the next required action
  • Continue using the hand probe to proceed
Programming Functions
6. Entering Program Mode
  • Press Program
  • Display shows: Enter/Edit Program # (next available slot blinks)
7. Editing or Deleting a Program
  1. Enter the program number → press Enter
  2. Choose:
    • Edit → modify steps
    • Delete → permanently remove program

Notes:

  • Edit mode: Step-by-step prompts appear
  • Press Enter to confirm each value
  • To change program structure, delete and recreate
8. Creating a New Program
  1. Press Program
  2. Accept the suggested program number → press Enter
    • OR enter a different number → press Enter
  3. Display shows: “New Program”
9. Optional: Linear Regression Control

For sub-microliter accuracy:

  • Select Slope Adjust ON → press Enter
  • Otherwise select OFF
10. Programming Steps

Available functions:

  • ReTip
  • Aspirate (Asp)
  • Dispense (Disp)
  • Blowout (Blast)
  • Rinse
  • Other
How to configure:
  1. Use Select to scroll functions
  2. Press Enter to choose
  3. Follow prompts for volumes and speeds
11. Important Controls
  • Enter: Confirms selections
  • Clear: Deletes incorrect input (NOT a reset)
Summary
  • Simple operation with familiar pipetting workflow
  • Step-by-step guided execution
  • High precision, even at sub-microliter volumes
  • Flexible programming for custom workflows

Our new dolphin nose mandrels, equipped with O-ring fittings and compatible with Labcon 8010 tips, support a high capacity of up to 50 µL. Differential mode enables precise aspiration at volumes as low as 1.5 µL.

In non-differential mode, liquid can be dispensed contact-free from several millimeters above the target surface. The tips do not need to touch the receiving surface, eliminating hanging droplets and ensuring clean, consistent delivery.

This type of run became routine by the end of the second day. It leveraged the large capacity of the tips by first dispensing 20–30 µL, followed by indexing the plate and dispensing approximately 1 µL. The results demonstrate consistent and controlled variation in droplet size

No hanging drops.

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